Route 20, or Highway 20, may refer to:

Canada

• Manitoba Highway 20
• New Brunswick Route 20
• Highway 20 (Ontario)
• Quebec Autoroute 20

Israel

• Ayalon Highway

Japan

• Route 20 (Japan)

United States

• Interstate 20
• U.S. Route 20
• State Route 20 (California)
• Route 20 (Connecticut)
• Delaware Route 20
• State Road 20 (Florida)
• State Route 20 (Georgia)
• Louisiana Highway 20
• Maryland Route 20
• Route 20 (Massachusetts)
• Michigan State Highway 20
• Minnesota State Highway 20
• Route 20 (Missouri)
• Route 20 (New Jersey)
• North Carolina Highway 20
• North Dakota Highway 20
• Ohio State Route 20
• State Highway 20 (Oklahoma)
• Tennessee State Route 20
• State Highway 20 (Texas)
• State Route 20 (Washington)
• State Route 20 Spur (Washington)
• West Virginia State Route 20
• Wisconsin Highway 20